ScholAR Chemistry has provided a useful reference guide for school laboratories. Information in the guide includes:
- cleaning and sterilizing glassware
- cleaning microscopes
- pH Defined
- making a buffering solution
- preparing molar & normal solutions
- dilution table for liquids
- useful reagents
- common preservatives and fixatives
- general stains
- Centigrade - Fahrenheit conversions
- Mohs’ Scale of Hardness
- weight, length and volume conversions
- table of atomic symbols, numbers and weights
Most of the reference material is included below for quick, easy access. However, conversion charts and other large tables are available in the PDF file linked below:
Ward’s ScholAR Lab Reference Guide (PDF, 60 kb)
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This overview of slide staining originally appeared on wardsci.com.
The exact chemistry involved in most staining procedures is unknown. There is probably more than one type of chemical interaction occurring during any staining procedure. Interactions can be electrostatic, covalent, or based on physical properties that affect stain penetration, including texture, density and solubility.
One prevalent mechanism is thought to be the electrostatic interaction of the chromogen with tissue molecules leading to the classification of many stains as acidophilic or basophilic. Basic dyes color acidic molecules - for example, hematoxylin, stains DNA (an acid) dark blue. Acid dyes color more basic molecules - for example, eosin, makes most cytoplasms red/pink. Continue reading ‘Slide Stain Guide’
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